Plasmid
Part:BBa_K1453003:Design
Designed by: Ziyang Tan, Renhe Luo Group: iGEM14_SJTU-BioX-Shanghai (2014-10-02)
pBluescript II KS(+)_3_copy
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 702
Illegal PstI site found at 696 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 702
Illegal PstI site found at 696 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 702
Illegal BamHI site found at 684 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 702
Illegal PstI site found at 696 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 702
Illegal PstI site found at 696
Illegal NgoMIV site found at 328 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2107
Illegal SapI site found at 1024
Design Notes
pBluescript II KS(+) ScaI deletion
Source
pBluescript II KS(+) ScaI deletion